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Product Usage Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Chronophin/PDXP (C85E3) Rabbit mAb detects endogenous levels of total chronophin/PDXP protein.

Species Reactivity:

Human, Mouse, Rat, Hamster, Monkey, Bovine

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant mouse MBP-chronophin.

Background

Cofilin is a conserved actin-severing protein required for processes that rely on actin dynamics, including cytokinesis and cell motility (reviewed in 1). Regulation of actin dynamics requires the controlled cycling between the phosphorylated and unphosphorylated forms of cofilin (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at the conserved amino-terminal Ser3 of cofilin (3,4). Slingshot (SSH) phosphatase, for which there have been three mammalian isoforms identified, dephosphorylates cofilin in vivo (5). Chronophin (CIN, PDXP) is a haloacid dehalogenase phosphatase that also dephosphorylates cofilin. Alteration of CIN activity through overexpression of either the wildtype or phosphatase-inactive mutant CIN interferes with actin dynamics, cell morphology and cytokinesis (6).

1. Carlier, M.F. et al. (1999) J. Biol. Chem. 274, 33827-33830.
2. Huang, T.Y. et al. (2006) Curr Opin Cell Biol 18, 26-31.
3. Arber, S. et al. (1998) Nature 393, 805-809.
4. Toshima, J. et al. (2001) J Biol Chem 276, 31449-58.
5. Niwa, R. et al. (2002) Cell 108, 233-246.
6. Gohla, A. et al. (2005) Nat Cell Biol 7, 21-9.