欧美gv在线观看,黑人97人人模人人爽人人喊,各类老熟女老熟妇视频在线观看 ,真人做爰高潮视频免费

Product Usage Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

IRS-1 (D23G12) Rabbit mAb detects endogenous levels of total IRS-1 protein. The antibody does not cross-react with IRS-2.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Ser270 of human IRS-1.

Background

Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10).

1. Sun, X.J. et al. (1991) Nature 352, 73-7.
2. Sun, X.J. et al. (1992) J Biol Chem 267, 22662-72.
3. Myers, M.G. et al. (1993) Endocrinology 132, 1421-30.
4. Wang, L.M. et al. (1993) Science 261, 1591-4.
5. Rui, L. et al. (2001) J Clin Invest 107, 181-9.
6. Gao, Z. et al. (2002) J Biol Chem 277, 48115-21.
7. Horike, N. et al. (2003) J. Biol. Chem. 278, 18440-18447.
8. Ozes, O.N. et al. (2001) Proc Natl Acad Sci U S A 98, 4640-5.
9. De Fea, K. and Roth, R.A. (1997) Biochemistry 36, 12939-47.
10. Li, Y. et al. (2004) J Biol Chem 279, 45304-7.